agarose
1. Typical DNA ladder was shown with 1.5% agarose gel electrophoreais.
1.5%琼脂凝胶电泳呈现典型的阶梯现象。
2. 1.2.3 RT-PCR reaction and agarose gel electrophoresis: take forementioned reversion record reaction product to 25 l reactant intermediate to proceed PCR reaction.
2.3 RI’- pcr反应和琼脂糖凝胶电泳:取上述逆转录反应产物于25川反应体
3. The significant of full automatic hemoglobin agarose electrophoresis with the analysis of 2000 Cases
2000例全自动琼脂糖凝胶电泳检测血红蛋白异常结果分析
4. Application of Helena SAS-3 full automatic hemoglobin agarose electrophoresis
3全自动琼脂糖电泳仪在血红蛋白分析中的应用及评价
5. Agarose Gel Electrophoresis Staining WithNBT for AST Isoenzvme Measurement.
AST同工酶的琼脂糖凝胶电泳NBT显色法
6. Results The agarose electrophoresis showed that the fragments of BMP-2, pGEM-T and pcDNA3.1(+) were 1.2 kbp, 4.0 kbp and 5.0 kbp, respectively.
pcDNA3.1-hBMP-2质粒经酶切电泳可见1.2kbp的hBMP-2条带和5.0kbp的载体片断,重组质粒酶谱分析与预期一致;
7. Agarose gel electrophoresis analysis of DNA extracted from dealled cell showed typical DNA ladder. All these supported that UVC treatsed-SL-1 cells undergo apoptosis.
SL-1细胞处理后DNA琼脂糖凝胶电泳成典型凋亡细胞DNA梯形电泳谱带。
8. A modified formaldehyde- agarose gel electrophoresis
一种改进的甲醛-琼脂糖凝胶电泳
9. A Simple, Efficient and Economic Method for Recovering DNA from Agarose Gel
一种简便、高效、经济的从凝胶中回收DNA的方法
10. Effect of CS-B on DRG neurite extension in three and two dimensional agarose gel culture
不同结合状态硫酸软骨素多糖链对体外二维三维培养背根节神经突起生长影响研究
11. Ready- to-run agarose electrophoresis system with double chamber
两槽式快速电泳槽
12. Keywords Lactate dehydrogenase isoenzyme;Agarose gel;Electrophoresis;
乳酸脱氢酶同工酶;琼脂糖凝胶电泳;
13. cross-linked agarose gel
交联琼脂糖凝胶
14. Our study indicated that the plasmid DNA of Shigella DNA abstracted from Shigella was determined with agarose gel electrophoresis, and conducted into E. Coli K_(12) W_(1485), which expressed antagonism on streptomycin.
从痢疾杆菌D_(15)提取质粒DNA,用琼脂糖凝胶电泳检测,并把它导入大肠杆菌E、Coil K_(12) W_(1485)。
15. In this test ,young rats drink alcohol of different concentration.Sixty days later,we extract DNA in epithelial cell of middle duodenum,examinate apoptosis of DNA through agarose gel electrophoresis.
以幼龄小白鼠为研究对象,使其饮用不同浓度酒精,然后提取十二指肠中段上皮细胞DNA,经琼脂糖凝胶电泳,检测细胞凋亡情况。
16. Aqueous two-phase system composed of PEG-Na_(2)SO_(4) was used to extract agarose and pepsin in this paper.
以聚乙二醇/硫酸钠双水相体系分离提取琼脂糖和胃蛋白酶。
17. DNA fragmentat ion,DNA agarose gel electrophoresis were used to observe the apoptosis of LP L an d IEL.
伤后6hLPL、IEL基因组DNA电泳均可见凋亡特征性梯形条带。
18. Keywords low energy laser;low density lipoprotein(LDL);agarose gel-electrophoresis;
低密度激光;LDL;血清脂蛋白琼脂糖凝胶电泳;
19. Low melting point agarose
低熔点琼脂糖
20. low melting-temperature agarose
低熔点琼脂糖(分)
21. PREIRRADIATION GRAFTING OF BSA NASI CONJUGATES ON AGAROSE AND ITS APPLICATION
偶合物BSA-NASI在琼脂糖上预辐射接枝及其应用
22. Keywords Microcarrier;Agarose;Cell culture;
关键词琼脂糖;微载体;细胞培养;
23. Keywords agarose film coated glass slides;Protein microarray;TORCH infectious;fluorescence dectection;
关键词琼脂糖;蛋白质芯片;荧光检测;TORCH感染;
24. Keywords tumor cell;soft agarose culture;differentiation;drug-resistant cell clone for chemotherapy;
关键词肿瘤细胞;软琼脂培养;分化;化疗耐药克隆;
25. The purified OGT fusion protein displayed as a single band in SDS-polyacrylamide gel and had enzymatic activity, but the activity lost after it was eluted from the agarose beads.
其纯化的 OGT在电泳中显现单一条带并具有生物活性 ,但其活性在洗脱后丧失。
26. The results show that the main constituent of each sample is unsubstituted agarose.
凝固温度与甲氧基的取代位置及含量有关;
27. Results showed the CM4 can inhibit the migration of DNA and RNA.With the increasing of the quantity of peptides, the moving speed of nucleic acids in agarose became slower.
凝胶阻滞实验证明,抗菌肽能与真菌的DNA和RNA结合,影响了它们在电泳中的迁移率,同时还能使核酸受到破坏,断裂为大小不等的片断。
28. Laminaria polysaccharide sulfate was degraded by free radical, then isolated and purified through agarose gel electrophoresis and gel chromatography.
分别利用硫酸酚法和明胶比浊法,测定糖的含量和硫酸根的含量;
29. Meyers JA,Sanchez D,Elwell LP,et al.Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.J Bacteriol,1976,127:1529.
华川,府伟灵,华岩,等.老年病房铜绿假单胞菌感染质粒暴发分析[J].中华医院感染学杂志,1999,9(4):211.
30. Keywords aqueous two-phase system;pepsin;polyethylene glycol;agarose;extraction;
双水相;胃蛋白酶;聚乙二醇;琼脂糖;萃取;
31. Antisense ECE was defined at the constant concentration of 1 g/100 L and 10 g/L agarose gel electrophoresis at 60 V for 1 hour.
反义内皮素转换酶核酸的浓度恒定为1g/100L,10g/L琼脂糖凝胶电泳60V电泳1h。
32. A Comparative Analysis on Staining Eff Iciencies of Four Fluorescent Dyes in Preparative Agarose Gel Electrophoresis
四种核酸荧光染料在琼脂糖凝胶中染色特性的比较分析
33. And plasmid profiles were clear respectively on agarose gel (0.5%), which were named P1,P2, P3 and P4 by their molecular weights.
在0.5%琼脂糖凝胶电泳中,质粒DNA分离相当清晰。 Bt 4.0718菌株含有4个大小不等的质粒,依据其分子量大小顺序依次编号为P_1、P_2、P_3和P_4。
34. In the process of “gel electrophoresis,” these biomolecules are put into wells of a solid matrix typically made of an inert substance such as agarose.
在凝胶电泳过程中,生物分子被注入由琼脂糖等惰性物质构成的固体胶块的孔里。
35. This is then coated with agarose, a nutrient gel that bacteria will grow on.
在印章上图一层琼脂糖,细菌就能够在这种营养凝胶体上生长。
36. agarose vertical slab gel
垂直琼脂糖软膏凝胶
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38. FACS analysis showed that monocytes express PAR-1, PAR-3, PAR-4, but not PAR-2. An agarose gel electrophoresis revealed that monocytes express PAR-1 and PAR-3, but not PAR-2 and PAR-4 mRNAs.
实验显示,人外周血单核细胞表达PAR-1、PAR-3和PAR-4但不表达PAR-2蛋白质,单核细胞表达PAR-1和PAR-3,但是不表达PAR-2和PAR-4 mRNAs。
39. Boil 1g Agarose in 100ml Gel running buffer (microwave: 45sec, swirl, 20sec, swirl, 20sec). Let cool for 10min.
将1克的琼脂洋菜粉末加入100毫升的胶体电泳缓冲液并煮沸(微波炉:45秒,晃几下,20秒,晃几下,20秒)。等10分钟待其降温。
40. Boil1 g Agarose in100 ml Gel running buffer( microwave:45 sec, swirl,20 sec, swirl,20 sec). Let cool for10 min.
将1克的琼脂洋菜粉末加入100毫升的胶体电泳缓冲液并煮沸(波炉:45秒,晃几下,20秒,晃几下,20秒)等10分钟待其降温。
41. Keywords mice;thymus;microcapsules;agarose;lupus erythematosus;systemic;
小鼠;胸腺;微胶囊;琼脂糖;红斑狼疮;系统性;
42. Keywords Urine protein;Agarose gel elctrophoresis;Sodium dodecyl sulphate;Kidney disease;
尿蛋白;琼脂糖凝胶电泳;十二烷基磺酸钠;肾脏病;
43. It can be measured the expression of c jun and c fos by using the methods of RT PCR and agarose gel electrophoresis.
应用RT PCR和琼脂糖凝胶电泳方法测定c jun和c fos基因的表达。
44. Detection of the apoptotic cells of lens epithelial from senile cataract by Tunel and DNA Agarose gel electrophoresis
应用原位末端转移酶标记和DNA电泳检测白内障晶体上皮细胞凋亡
45. Detection of Erythrocyte Isoenzymes EsD and GLOI Phenotypes Using a Mixed Starch Agarose Gel Electrophoresis
应用淀粉/琼脂糖混合凝胶电泳同步检测红细胞EsD和GLOI表型
46. Research on Screening of the Antitumor Drugs and Obtaintion of Drug-resistant Clone Cell by Soft Agarose Culture
应用软琼脂培养法筛选抗癌药物及获得耐药克隆细胞的研究
47. Keywords Microarray;Agarose;Antigen;Autoantibody;characterization;
微阵列;琼脂糖;抗原;自身抗体;表征;
48. A Phenomenon of Ring-like Precipitum Formed by Diffusing Sera of Mice Infected with Plasmodium berghei in Agarose Gel
感染伯氏疟原虫小鼠血清在琼脂糖凝胶上扩散形成沉淀环的现象
49. Keywords Bay scallop (Argopecten irradiaus );glycosaminoglycan;agarose gel elec-trophoresis;analysis;
扇贝;糖胺聚糖;琼脂糖凝胶电泳;分析;
50. The amplicons can be separated by polyacrylamide gel, denaturing polyacrylamide gel or agarose gel, and detected by autoradiography, silver or EB staining.
扩增产物可在聚丙烯酰胺凝胶、变性聚丙烯酰胺凝胶、琼脂糖凝胶上进行电泳分离,检测时可使用同位素、银染或溴化乙锭染色等技术。
51. The amplicons can be separated by polyacrylamide or agarose gel,and detected by autoradiography,silver or EB staining.
扩增产物可在聚丙烯酰胺或琼脂糖凝胶上电泳,同位素或银染、EB检测。
52. PCR products were examined on a agarose gel, which full-length was 987bp.
扩增产物在1%琼脂糖凝胶中长度为987bp。
53. A PET32a vector containing human OGT cDNA-S tag was expressed in BL21 E. Coli and the recombinant protein was purified with S-protein conjugated agarose beads.
携带人 OGTc DNA的 PET32 a质粒在大肠杆菌中表达出带有 1个 S- Tag的重组人 OGT融合蛋白 ,然后用与 S-蛋白质偶联的琼脂糖凝胶颗粒从细菌裂解液中亲和纯化 OGT融合蛋白。
54. Apoptosis and the tolerance of plantlets of Acacia implexa treated in vitro with cold stress were studied by microphotographics and total DNA agarose gel electrophoresis.
摘要利用显微观察和DNA凝胶电泳等技术,研究在低温胁迫诱导下灰木相思组培苗细胞的凋亡和对低温环境的耐受性。
55. Application of alkaline phosphatase separated by improved agarose electrophoresis in the diagnosis of renal diseases
改良琼脂糖电泳法分离碱性磷酸酶同工酶及其在肾脏疾病诊断中的应用
56. Methods: A fragment 287bp Alu sequence in intron 16 of ACE gene was used as insertion/deletion(I/D) polymorphic marker. After PCR amplification of DNA fragment, 1% agarose gel electrophoresis was undertaken to analyze the PCR products.
方法 :以 ACE基因第 16内含子 1个 2 87b P Alu顺序插入 /缺失 (I/D)型为多态性标志 ,用聚合酶链反应 (PCR)方法扩增 ACE基因第 16内含子片段 ,1%琼脂糖凝胶电泳分析 PCR产物。
57. Method:Four groups of buffers were choiced to made 0 7% of agarose and adding 1% Triton X-100 in it. For treating samples with neuraminidate before electrophoresis,then the samples were scanned by fluoresce.
方法 :将血清用神经氨酸苷酶处理 ,在此基础上选用四种缓冲液配制成 0 7%的凝胶 ,加入 1%的tritonX - 10 0电泳 ,并进行荧光扫描。
58. Methods:Lipoproteins were separated by agarose gel electrophore- sis. The TG band was stained by specific enzyme (GPO- Trinder) thereafter.
方法 :通过脂蛋白电泳分离脂蛋白 ,用 TG酶试剂 ( GPO- Trinder反应法 )与电泳后区带中 TG反应而呈色。
59. Methods The MT1 MMP hTAP protein complexes were isolated and purified using the IgG agarose beads and calmodulin sepharose beads affinity chromatography according to the molecular and biological features of TAP system.
方法 应用TAP系统分子生物学特性采用IgG和钙调蛋白亲和胶粒二次亲和层析法分离纯化MT1 MMP hTAP蛋白复合物。
60. Methods:To analysis serum electrophoretograms of expectant mothers and other patients with nephronia,hepatopathy,mutiple myeloma etc by agarose electrophoresis.
方法:采用国产全自动琼脂糖电泳系统对待产妇女和肾病、肝病、多发性骨髓瘤等患者新鲜血清标本进行血清蛋白电泳分析。
61. Methods:ALP isoenzymes in the serum of normal persons and tumor patients had been separated by agarose isoelectric focusing(IEF).
方法:采用神经氨酸苷酶对正常人及患者血清中ALP同工酶进行处理后进行琼脂糖等电聚焦。
62. Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data.
方法优化RAPD及ISSR的PCR反应体系,对产物琼脂糖凝胶电泳结果进行数据统计。
63. Methods The inhibition activity on P.gingivalis,P.intermedia and F.nucleatum of S.sanguis suspension and supernatant were measured by Pucci test and Agarose gel diffusion method.
方法分别采用交叉双层培养基法和微量琼脂糖弥散法测定血链球菌全菌细胞和培养上清液对牙龈卟啉单胞菌、中间普雷沃菌、具核梭杆菌的拮抗作用。
64. Methods The polymorphisms of DYS287 locus in male 105 Tibetans(41 in Lhasa and Individuals Nagqu)were analyzed by PCR and agarose gel electrophoresis.
方法利用PCR结合琼脂糖凝胶电泳检测方法对105例藏族男性样本(其中拉萨41例,那曲64例)DYS287位点多态性进行分析。
65. Methods The genomic DNA was analyzed by agarose gel electrophoresis and absorbance detection method and PCR amplification was carried out for further comparison.
方法对白花蛇舌草不同部位提取的基因组DNA进行PCR扩增后,采用琼脂糖凝胶电泳和吸光度测定进行比对分析。
66. Method According to the difference of the isoelectric point (pI) of LDH isoenzymes, and selecting agarose gel as sustaining material,the 5 type isoenzymes were seperated and determined.
方法据LDH同工酶等电点不同,选择琼脂糖凝胶作支持物,对以往LDH同工酶测定方法进行改进,将五型LDH同工酶进行分离并定量测定。
67. Methods ALP isoenzymes in the serum of normal healthy and 162 renal patients were separated quantitatively by improved agarose electrophoresis.
方法用改良琼脂糖电泳法对正常体检者和162例肾脏疾病患者碱性磷酸酶(ALP)同工酶进行分离和定量分析。
68. Methods Separated by neuraminidase,ALP isoenzyme in the serum of 50 normal women and 55 senile women with osteoporosis were analyzed by agarose electrophoresis.
方法采用神经氨酸苷酶,用琼脂糖电泳法对体检正常者50例(对照组)和老年女性骨质疏松患者55例(骨质疏松组)血清中的ALP同工酶进行分离分析。
69. Methods Polymerase chain reaction(PCR)and 2% agarose gel electrophoresis were used to analyze the genetic polymorphism of 334 unrelated males from the six ethnic populations.
方法采用聚合酶链式反应(PCR)和琼脂糖凝胶电泳检测方法,对上述六群体334份男性血样本的遗传多态性进行分析。
70. Methods The Helena spife 2000 agarose gel electrophoresis technique was used for serum protein electrophoresis in both 90 pregnant women(study roup)and 30 healthy unpregnant women(control group).
方法采用英国helena Spife2000蛋白电泳仪测定90例待产妇(实验组)、30例育龄非孕健康女性(对照组)的血清蛋白电泳。
71. Methods BALB/c mice and rabbits were immunized with rUreB.The specific antibodies to UreB were analyzed by agarose diffusion, ELISA and Western blot.
方法重组尿素酶B亚单位注射免疫BALB/c小鼠、家兔,双向琼脂扩散、ELISA、免疫印迹分析特异抗体的产生及性质。
72. Method:The DNA gradent bands were detected by agarose gel electrophoresis.
方法:用琼脂糖凝胶电泳法观察DNA的梯状条带。
73. electroendosmosis-free agarose gel
无电渗性琼脂糖胶
74. The results of EAP phenotyping of Chinese population(N = 523) in Guangzhou area, using the agarose gel electrophoresis, were presented.
本文介绍用琼脂糖凝胶电泳法对广州地区523例健康无关个体EAP表型的检测结果。
75. Cleavage action of pBR322 DNA by bleomycin(BLM), bleomycin A 5(BLMA 5 ) and peplomycin (PEP) was determined by electrophoretic analysis in agarose gel.
本文用琼脂糖凝胶电泳分析测定了博莱霉素(BLM),平阳霉素(BLMA_5)和匹莱霉素(pEP)对pBR322 DNA的断裂作用。
76. L. E. Olssen, S Petersson, B. A. Westrin, A. Fransson and B. Nordell, “Diffusion of Ferric Ions in Agarose Dosimeter Gels,” Phys. Med. Biol., 37, 2243-2252, 1992.
杨左元,“硫酸亚铁剂量胶之铁离子扩散效应探讨,”国立阳明大学医学工程研究所硕士论文,民国九十一年六月.
77. DNA Staining Efficiencies of Different Fluorescent Dyes in Preparative Agarose Gel Electrophoresis: A Comparative Analysis
核酸荧光染料在琼脂糖凝胶电泳中的染色特性
78. Keywords He-Ne laser;low density lipoprotein(LDL);agarose gel electrophoresis;
氦氖激光;低密度脂蛋白;血清脂蛋白琼脂糖凝胶电泳;
79. agarose horizontal slab gel
水平琼脂糖软膏凝胶
80. The oxidative modification of LDL was identified by agarose gel relative electrophoretic mobility(REM)and fluorescence of thiobarbituric acid reaction substances(TBARS)contents.
测定LDL的电泳迁移率及硫代巴比妥酸反应物质含量。