coomassie

coomassie

1. 3. (4) Staining: with silver and coomassie brilliant blue staining.

(4)染色：行银染和考马斯亮蓝染色；

2. The determination of 24 hours urine protein? using coomassie light blue technique.

24h尿蛋白测定:采用考马斯亮蓝法。

3. 3)gels were stained with Coomassie blue and changes were compared between treated- and untreated-groups,those spots with dominant differences were selected;

3) 考马斯亮蓝染色,在PDQuest软件的辅助下进行实验组与对照组蛋白质表达的比较,切取差异点。

4. In addition, the staining of mPEG with BaCla and la is more specific and sensitive than the Coomassie Brilliant Blue staining.

SDS-PAGE采用BaCl_2和I_2对mPEG染色比考马斯亮蓝染色水蛭素更为灵敏，特异性也很高。

5. A Simple and Easy, Low Background Combined Coomassie Blue-Silver Staining Method

一种简易的与考马斯亮蓝相结合的低背景银染法

6. Cx36 also bound to PDZ1 domain of ZO-1 in mouse retina and Cx43 bound to PDZ2 domain of ZO-1 in mouse brain. Fusion protein was detected in the membranes with anti-GST and coomassie blue.

与鼠视网膜组织孵育免疫印迹显示Cx36与ZO-1的PDZ1结构域结合,而对照组Cx43与ZO-1的PDZ2结构域结合,相同膜洗脱后抗GST抗体和考马斯亮蓝染色显示GST-PDZ融合蛋白表达。

7. OUTER MEMBRANE PROTEIN (OMP) PATTERNS OF ESCHERICHIA COLI ISOLATES OF CHICKEN ORIGIN VISULIZED WITH COOMASSIE BRILLIANT BLUE STAINING AND SILVER STAINING

两种染色法测定禽源性大肠杆菌分离株的外膜蛋白型

8. The structural characteristics of the microfilaments were revealed in sterile pollenes of CMS, CHA and mature fertile on wheat by Coomassie brilliant blue R250.

以T型细胞质雄性不育(CMS)、化学杂交剂(CHA)诱导的雄性不育及成熟可育的花粉粒为材料,用考马斯亮蓝R250显示细胞内微丝的方法,观察分析了3种类型花粉粒内微丝结构的变化。

9. BSA was run on SDS- PAGE, stained with Coomassie Brilliant Blue(CBB) G- 250, or reverse stained with Imidazole- Zinc, then recovered by using electroeluter.

以牛血清白蛋白(BSA)为材料进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),分别采用考马斯亮蓝(CBB)G-250染色和咪唑-Zn反染,用电洗脱仪回收。

10. Catalytic Spectrophotometric Determination of Trace Manganese with Coomassie Brilliant Blue G

催化NaIO_4氧化考马斯亮蓝G褪色光度法测定痕量锰

11. Keywords Catalytic spectrophotmetry;Iron;Coomassie brilliant blue G;

催化反应;光度法;铁;考马斯亮蓝G;

12. Keywords Resonance light scattering;Coomassie brilliant blue;Protein;Sodium dodecanesulphonate;

共振光散射光谱;考马斯亮蓝;蛋白质;十二烷基磺酸钠;

13. Appearence, location and arrays of cortical microtubules are consistent with those of cellular network visualized by staining of Coomassie brilliant blue R250.

其出现时间、布状况以及排列形态,基本上与考马斯亮蓝染色法观察到的网状结构物相一致。

14. SDS-PAGE Coomassie brilliant blue microwave staining

凝胶电泳考马斯亮蓝微波染色

15. 7Kang DH，Gho YS，Suh MK. Highly sensitive and fast protein detection with Coomassie brilliant blue in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bull Korean Chem Soc，2002；23: 1511-1152

几种双向凝胶电泳蛋白质检测方法的比较由800论文网收集整理，转载请注明出处！

16. Keywords method of Micro-Kjeldahl;Coomassie brilliant blue;alkaline copper carbonate;protein in the grain of black rice;pigment;

凯氏定氮法;考马斯亮蓝;碱式碳酸铜;黑稻蛋白;色素;

17. Keywords spectrophotometry;coomassie brilliant blue G250;cationic surfactants;cetylpyridinium bromide;cetyl trimethyl ammonium bromide;tetraethyl ammonium bromide;tetrabutyl ammonium bromide;

分光光度法;考马斯亮蓝G250;阳离子表面活性剂;溴化十六烷基吡啶;溴化十六烷基三甲基铵;溴化四乙基铵;溴化四丁基铵;

18. 500 spots on the gels with a pH3-10 gradient and 600 spots on the gels with a pH4-7 gradient were observed, respectively, when the gels were stained by Coomassie blue and analyzed by ImageMaster~(TM)2D Platinum software (version 5.0).

利用pH3-10和pH4-7两种不同pH梯度胶条,经考马斯亮兰染色显示和ImageMaster TM 2D Platinum software (version 5.0)软件分析后,在双向电泳凝胶上各自检测到500和600个左右的蛋白点。

19. The cyst fluid showed 10 bands in PAGE and 16 bands in SDS-PAGE stained by Coomassie brilliant blue R-2SO, one band stained by PAS and another by Sudan black B. 14 IE antigenic components in cyst fluid were demonstrated.

囊液抗原有10条(PAGE)和16条(SDS-PAGE)考马斯亮蓝染色带、PAS和苏丹黑B染色带各1条,免疫学抗原14种。

20. Silver staining was fit to ascertain protein spot in 2-DE gels, coomassie blue was fit to detect high content protein, DIGE was fit to establish difference protein expression map.

在三种蛋白质的检测方法中，硝酸银染色适合确定蛋白质点的有无，考马斯亮蓝R-250染色适合目标蛋白质为高丰度且样品量大的情况，DIGE技术适合用于差异蛋白质表达谱的分析。

21. In weak acidic medium, sodium periodate can oxidize Coomassie brilliant blue G and cause discoloring of coomassie brilliant blue G, and manganese can catalyze this oxidizing reaction.

基于弱酸性条件下高碘酸钠能氧化考马斯亮蓝G褪色,锰对此褪色反应具有显著的催化作用,由此建立了催化高碘酸钠氧化考马斯亮蓝G褪色光度法测定痕量锰的新方法。

22. Above-mentioned expressed protein(about 14ku)amount to 34.46mg/L according to Coomassie Brilliant;

大肠杆菌表达产物经SDS-PAGE电泳检测到相对分子量约为14ku的目的蛋白； 用考马斯亮兰法测定所表达的外源蛋白表达量为34.46mg/L；

23. The method was applied to the determination of the content of HSA in blood samples and the results were in good accordance with those of the traditional Coomassie Brilliant Blue G-250 spectrophotometric assay.

将本法应用于人血清样品的测定所得结果同考马斯亮蓝G-250光度法一致。

24. It is used for the determination of total protein in urine and human serum samples, the rusult obtained by this method agreeds well with those obtained by Coomassie brillant blue G?250 method.

并且方法选择性好,用于尿液及人血清样品中总蛋白的测定,所得结果与考马斯亮蓝法基本一致.

25. The results were compared with traditional method(Coomassie Brilliant Blue R250).

并和考马斯亮蓝R250染色方法比较。

26. Coomassie is a city of south-central Ghana northwest of Accra. Founded c.1700,it is a commercial and transportation center in a cocoa-producing region. Population,348,880.

库马西是加纳中南部一城市，位于阿克拉西北。建于1700年左右，地处盛产可可的地区，是一个商业和运输中心。人口348，880。

27. By using isoelectric focusing(IEF) method,eggs,nymphs and adults of Blattella germanica showed 36,30 and 43 bands of proteins respectively through coomassie brilliant blue stain,and 29,21 and 33bands of sugar through PAS stain.

德国小蠊生活史各期经等电聚焦后，用考马斯亮兰染蛋白质，然后用薄层层析扫描仪扫描，卵、若虫、成虫分别显带36、30和43条； 用PAS染糖，分别显带29、21和33条。

28. The proposed method based on this binding reaction,has been used to the determination of total proteins in human serum samples with satisfactory results in agreement with those of the coomassie brillant blue G-250(CBBG-250)method.

所拟方法用于人血清中总蛋白的测定 ,结果与考马斯亮蓝G_250法一致

29. Keywords arch expansion;age;chondroid cell;Coomassie BB;compressive pressure;cyclic strain force;cytoskeleton;signal transduction;integrin;

扩弓;年龄;细胞骨架;整合素;细胞增殖;凋亡;信号传导;机械力;

30. It involves the binding of Coomassie Brilliant blue to protein。However, detergents such as sodiu...

摘要：The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration。

31. Methods:The BSA standard protein was incised from 2-DE gel by Coomassie Brilliant Blue dyeing and was mixed with matrix (CHCA),then analyzed by MALDI-TOF MS.

方法:从考马斯亮蓝染色的2-DE胶上取下牛血清白蛋白(BSA)标准蛋白进行胶内原位酶切,与基质(CHCA)混匀后进行MALDI-TOF MS分析;

32. MethodsAfter gelatin-PAGE and gelatin-SDS-PAGE,the gel was incubated in Tris-HCI buffer,containing trypsin or chymotypsin,and then was stained with Coomassie blue R250.

方法材料经过明胶-PAGE和明胶-SDS-PAGE后,分别用胰蛋白酶进行酶解,考马斯亮兰染色。

33. Methods The human serum albumin was removed by Hitrap Blue column,and serum was dealed with U9.Then gel was stained by the improved method(0.1% carbinol,0.5% TCA,0.1%Coomassie briliant blue G250).

方法用Hitrap Blue层析柱去除血清中白蛋白,用U9对血清样品进行变性处理,采用改进后的方法(0.1%甲醇,0.5%三氯乙酸,0.1%考马斯亮蓝G250)进行染色。

34. Methods The total proteins of hypothalamuses in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining,the 2-DE image was analyzed by PDQuest 7.0 software.

方法用固相pH梯度双向凝胶电泳分离肝阳上亢证大鼠与正常大鼠下丘脑的总蛋白质,经考马斯亮兰染色,PDQuest 7.0软件分析。

35. Method The Coomassie Brilliant Blue G250 was dissolved into the ethanol and phosphate,then the SDS-PAGE was stained with the solution.

方法用考马斯亮蓝G250和磷酸作为染色液,用水作为脱色液。

36. Methods Semen smears from 55 male infertility patients were stained according to Wright-Giemisa, Modified Papanicolaou and Coomassie brilliant blue methods.

方法：分别采用改良巴氏染色法、瑞-吉染色法和考马斯亮蓝染色法对55例男性不育症患者精液涂片染色，计算形态正常精子率和顶体完整率。

37. VSMCs were lysed and the concentration of protein was measured using Coomassie blue G250 staining technique. PDGFR- 3 protein expressions were determined by Western Blotting.

时，裂解细胞提取蛋白，考马斯亮蓝G一250染色法测定蛋白浓度，使用Westem Bloning法检测PDGFR一p蛋白表达量。

38. Protein crystals, large or small, can be stained with Mercury bromphenol blue, Amido black 10B and Coomassie brilliant blue R250 or G250 but salt crystals are not.

本文报导了蛋白质晶体能被汞溴酚蓝、氨基黑10B和考马斯亮蓝R250或G250染色,而无机盐晶体不被染色.

39. The results of determination for three serum samples were identical with those obtained according to the Bradford method using Coomassie Brilliant Blue (CBB G-250).

本方法成功应用于三个血清样品中蛋白质总量的测定，其结果与考马斯亮蓝法(Coomassie Brilliant Blue,CBB G-250)测定结果一致。

40. ?A new indicator reaction is developed on the basis of manganese catalyzed oxidation of coomassie brilliant blue G250 by potassium periodate with nitrilotriaetic acid as an activator.

本研究采用次氮基三乙酸作活化剂，锰催化高碘酸钾氧化考马斯亮蓝G250褪色的指示反应。

41. Staining: with silver and coomassie brilliant blue staining.

染色：行银染和考马斯亮蓝染色；

42. Detection of indexes: The 24-hour urine protein was detected with Coomassie brilliant blue method;

检测指标:考马斯亮蓝法检测24h尿蛋白含量;

43. All stylet-secretions can be stained with coomassie brilliant blue G-250 which specially dye protein, therefore, stylet-secretions must contain proteins.

此外，5个株系均对考马斯亮蓝敏感，而考马斯亮蓝是特异染蛋白的染料，因此，可证明口针分泌物中存在蛋白质。

44. Keywords biological probe;fluorescein dye protein system;coomassie brilliant blue;

生物探针;荧光染料蛋白质体系;考马斯亮兰法;

45. Hypothalamic total protein (TP) of rats in each group were separated by immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE), and PDQuest 7.0 software was applied to analyse 2-DE images after coomassie blue staining.

用固相pH梯度双向凝胶电泳分离各组大鼠下丘脑的总蛋白质,经考马斯亮兰染色,PDQuest7.0软件分析。

46. tritici to study the effect of fungicide onthe pathogenesis of powdery mildew fungus in the leaves of wheat.Theinfected leaves were examined by a rapid staining method using a protein-specific dye(Coomassie Brilliant Blue R-250).

用特种蛋白质染料柯马氏亮兰(Coomassie Brilliant Blue R-250)叶片快速透明染色法系统研究了锈粉宁对小麦白粉病菌侵染叶部过程的影响。

47. Un-adsorbed protein was detected by coomassie brilliant blue G-250 method.

用考马斯亮蓝G-250法检测未反应的蛋白质。

48. Method Research of Measuring Soluble Protein Contents of Animal Material Rough Extraction Using Coomassie Brilliant Blue

用考马斯亮蓝测定动物药材中可溶性蛋白质含量方法初探

49. Method research of measuring soluble protein contents of plant rough extraction using Coomassie Brilliant Blue

用考马斯亮蓝测定植物粗提液中可溶性蛋白质含量方法的研究

50. The reactions of saccharide with Anthron and phenol-sulphate acid are used to analyze saccharide content.The content of protein in the products is determined by Coomassie Brilliant Blue method.

用蒽酮比色法和酚-硫酸法测定产品糖含量，考马斯亮蓝法测定蛋白质含量。

51. It was shown that 21 electrobands with antigenic activities were identified by enzyme immunoassay (EIA), 12 ConA-binding glycoprtein electrobands by ConA bridge method, and only 7 protein bands by Coomassie brolliant blue G250 staining.

用转移电泳结合酶免疫分析测得21条具抗原活性电泳条带; 结合刀豆蛋白A(ConA)搭桥法测得12条能与ConA结合的糖蛋白电泳条带;

52. Keywords electrophoresis;staining method;Coomassie Brilliant Blue G250;

电泳;染色方法;考马斯亮蓝G250;

53. After silver staining or coomassie blue staining, images were captured by scanner, and then the images were edited and matched using Imagemaster2DE analysis software.

电泳后经银染或考马斯亮蓝染色，ImageMaster双向凝胶电泳软件分析，将有意义的差异蛋白质点胶内胰蛋白酶解，经过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)鉴定。

54. coomassie brilliant blue G250 - betahistine hydrochlonide sodium chloride system.

盐酸培他啶与考马斯亮蓝G250相互作用的反应体系;

55. Objectives: To evaluate the application of Coomassie brilliant blue( CBB) G250 staining for the detection of human sperm deformity rate, rate of intact acrosome and acrosome reaction.

目的:探讨考马斯亮蓝G250染色法在人精子畸形率、体完整率和顶体反应检测中的应用。

56. This study investigated effect of different concentrations of Cu~(2+) and Zn~(2+) on heat stability of SOD. The activity of SOD was measured and the content of protein was determined using coomassie brilliant blue G-250 method.

研究了不同的Cu2 + 和Zn2 + 浓度配比对SOD热稳定性的影响 ,并测定了SOD活性及利用考马斯亮蓝G 2 5 0法测定蛋白质含量。

57. The binding reaction of the dye coomassie brilliant blue with proteins have been studied.

研究了考马斯亮蓝与蛋白质的结合反应。

58. Coomassie dye

科麦西染料

59. Keywords laccse;Trametes gallic;coomassie brilliant blue;decolorization and gegradation;

粗毛栓菌;漆酶;考马斯亮蓝;染料的脱色降解;

60. Decolorization and degradation of coomassie brilliant blue G-250 by laccase from Trametes gallic

粗毛栓菌产漆酶对考马斯亮蓝的脱色降解

61. Keywords Sperm capacitication;Acrosome reaction;Pentoxifylline;Coomassie brilliant blue;

精子获能;顶体反应;己酮可可碱;考马斯量蓝;

62. The purified GAD showed two main bands,67 kD and 44 kD,on SDS-PAGE by Coomassie Brilliant Blue R-250 and Western-Blot under reducing condition.

纯化的GAD在变性条件下电泳,经考马斯亮蓝R2 5 0染色以及Western Blot鉴定主要有两条带,分子量分别为6 7kD和4 4kD。

63. The result revealed there were 38 and 36 bands respectively on the plate staining protein with Coomassie brilliant blue R-250;

结果表明,用考马斯亮蓝R-250染蛋白质分别显示38及36条带;

64. The total protein of tumor cell was detected by coomassie light blue G-250.Results: 1.The PCR cycles and template concentrations did not affect the determination of proportions of LDH-M cDNA to total LDH cDNA.

结果：1、PCR反应的循环次数和模板浓度对LDH-M亚基cDNA在LDH总cDNA中所占比例影响不大；

65. Coomassie breiliant blue

考马斯亮兰

66. Keywords Coomassie breiliant blue;Chitosan;Determination of concentration of protein;Spectrophotometry;

考马斯亮兰;壳聚糖;蛋白质含量测定;分光光度法;

67. Determination of protein in apple juice concentrate production by Coomassie Brilliant Blue G-250 Method

考马斯亮兰G-250法测定苹果浓缩汁生产中的蛋白含量

68. The Determination of Protein in Chitosan with Coomassie Breiliant Blue G-250

考马斯亮兰法测定壳聚糖中蛋白的含量

69. The influences of PAN on the total protein concentration were measured with coomassie brilliant blue and the effects of PAN on the expression of ERK1/2 were studied by western blotting analysis.

考马斯亮兰蛋白测定法检测PAN 对细胞总蛋白浓度的影响; Western Blotting 法观察PAN 对细胞外信号调节激酶(ERK1/2)蛋白表达的影响。

70. The gels were stained by Coomassie Brillaint Blue R-350 and scanned to the Dell workstation. The matching rate of protein and differential expression analysis of 2-DE diagram were conducted with Image Master 2D Elite v3.01 software.

考马斯亮篮R-350染色后,扫描至Dell工作站中,Image-Master2DElitev3.01软件对2-DE图谱进行蛋白质点匹配率及差异表达分析。

71. Coomassie brilliant blue

考马斯亮蓝

72. Determination of Protein with Coomassie Brilliant Blue by the Resonance Rayleigh Light Scattering Method Using SLS as Sensitizer

考马斯亮蓝-SLS体系共振瑞利散射法测定蛋白质

73. Keywords Coomassie Brilliant Blue;fresh milk;protein;

考马斯亮蓝;牛乳;蛋白质;

74. coomassie brilliant blue G

考马斯亮蓝G

75. Coomassie brillant blue G - 250

考马斯亮蓝G-250

76. Determination of Protein Contents of Radix Aconiti Kusnezoffii Using Coomassie Brillant Blue G-250 Dye Binding

考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量

77. Coomassie brilliant blue G250

考马斯亮蓝G250

78. Spectrophotometric Determination of Cationic Surfactants in Water with Coomassie Brilliant Blue G250

考马斯亮蓝G250光度法测定水样中阳离子表面活性剂

79. Keywords Coomassie brilliant blue G250 staining;Sperm morphology;Acrosome reaction;Human;

考马斯亮蓝G250染色法;精子形态;顶体反应;人;

80. Determination of Trace Iron by Catalytic Spectrophotometry with Coomassie Brilliant Blue G

考马斯亮蓝G动力学光度法测定痕量铁的研究