protoplast

protoplast

1. In 1972 , Dutch De Vessels split pleat bacterium protoplasm body by lyase for the first time. The protoplast of Agaricus bisporus and straw mushroom were split soon.

1972 年荷兰De Vessels首次用裂解酶分离了裂褶菌原生质体,随后又分离了双孢蘑菇和草菇的原生质体。

2. He Ne LASER TREATMENT OF THE PROTOPLAST FROM ASPERGILLUS NIGER Sx

He-Ne激光诱变黑曲霉Sx的原生质体

3. KM8P medium effectively reduced the browning tendency of the cells and was a good medium for protoplast culture of B. campestris ssp.

KM8P是结球白菜原生质体培养更适宜的培养基,能显著减轻细胞的褐化;

4. PEG-mediated protoplast transformation

PEG介导转化

5. The Strain Q4413 was obtained by the protoplast fusion between Bacillus subtilis BR151 derivative and Corynebacterium Pekinese 1134 derivative.

Q4413菌株系北京棒状杆菌1134衍生物与枯草芽孢杆菌151衍生物的原生质体的融合株。

6. OUYANG X Z,XIE S P,LI B J.Differentiation and ultrastructure of the protophloem seive elements of minor veins in the maize leaves:Changes in the protoplast[J].Acta Botanica Sinica,1998,40(1):14-21.

[4]欧阳学智,谢绍萍,李宝健.玉米叶片细麦原生韧皮部筛分子的分化和超微结构:原生质体的变化[J].植物学报,1998,40(1):14-21.

7. The technique for protoplast fusion of filamentary fungi

丝状真菌原生质体融合技术

8. The Theological System of Ancient Chinese Literature: Protoplast Theory

中国古代文学理论体系:原人论

9. The feasibility of the protoplast mutagenesis for increasing productivity was studied on a Claviceps paspali strain, which was not easy to produce conidia under laboratory conditions.

为了提高在一般条件下不易产生分生孢子的雀稗麦角菌的产碱率,对原生质体诱变育种的条件和方法进行了探讨。

10. The low cell division frequency of the protoplasts is one of the main problems i n potato protoplast manipulation.

为了提高马铃薯原生质体培养时的细胞分裂频率， 对原生质体游离、 纯化， 培养过程中 的几个重要环节进行了研究。

11. To improve the Ethanol Fermentation of Xylose of Pichia guillermondii and Candida shehatea,we uses the Protoplast Fusion and Mutagenisis to breed five better strains XX2, X1, X4, X12 and X13.

为提高季也蒙毕赤酵母和休哈塔假丝酵母代谢木糖生产乙醇能力,采用原生质体融合以及化学及物理诱变方法进行育种,得到五个乙醇产量较高的菌株XX2,X1,X4,X12和X13。

12. ADVANCES IN PROTOPLAST CULTURE OF THE IMPORTANT CEREAL CROPS

主要禾谷类作物原生质体培养研究进展

13. In this test, the protoplast regeneration of starting strains which they can product fusants was bigger than those of non-fusants.

从发生融合的几个亲本菌株来看，其再生率普遍高于未发生融合的亲本菌株。

14. The report studied the effects of IOA and R-6G on mitochondria inactivation of cotyledon protoplast of non-heading Chinese cabbage 91 HQiu -21 (Aijiaohuang).

以 91H秋 - 2 1(矮脚黄 )不结球白菜为试材 ,研究了碘乙酰胺 (IOA)和罗丹明 (R 6G)对子叶原生质体线粒体失活效果的影响。

15. Calli of D. sophia,pretreated in low temperature and dark for 24 h,were used to isolate protoplast for highest yield,formulated efficent protoplast-culturing system.

以4℃低温暗处理24 h的播娘蒿愈伤组织为分离原生质体的来源材料,采用B5、Km8p、MS 3种培养基进行液体浅层静置培养原生质体,获得了愈伤组织并分化成苗,建立了原生质体培养体系。

16. The inosine producing strain B3 was selected as the parent strain. Mutant B1916 with high D-ribose-producing ability was isolated by united mutagenic treatment of DES, uvradiation and protoplast treated by UV-way.

以肌苷产生菌B3为出发菌株 ,通过硫酸二乙酯、紫外线及原生质体紫外线等复合诱变处理 ,获得一株核糖高产积累突变株B1916。

17. On the Definitions of Protoplasm and Protoplast

关于原生质和原生质体的定义

18. Keywords brewer's yeast;protoplast fusion;fermentative character;pilot scale;

关键词啤酒酵母;原生质体融合;发酵特性;中试;

19. Title： Studies on plant regeneration from protoplast culture in rapeseed (Brassica napus L.

关键词：甘蓝型油菜;原生质体培养;培养基;植株再生

20. Title： PROTOPLAST CULTURE AND PLANT REGENERATION OF THE METHIONINE RESISTANT VARIANT OF ASTRAGALUS CICER L.

关键词：鹰嘴紫云英;甲硫氨酸抗性系;原生质体培养;植株再生

21. Test was done on inactivation of protoplast of P.ostreatus by temperature and duration.

其次进行了温度和时间对平菇原生质体灭活效果的研究。

22. In order to obtain the mutants of carotene-high-producing,the mutagenic effect of ions implantation on Rhodotorula glutinis NR06 and its protoplast were studied.

分别以红酵母NR06和其原生质体为研究对象,通过注入生命必需元素N+以期获得高产类胡萝卜素突变株。

23. Effect of Initial Culture Condition on Protoplast Culture of Cabbage and Radish

初期培养条件对甘蓝和萝卜原生质体培养效果的影响

24. Studies on Breeding of Wine Yeast with Good Ability of Reducing Acid by Single Inactivated Protoplast Fusion Technique

利用单灭活原生质体融合技术选育降酸能力强的葡萄酒酵母

25. Thirteen kinds of yellow and three kinds of white monokaryons were isolated from mycelium of eight Flammulina velutipes strains using the monokaryon protoplast technique.

利用原生质体单核化技术从8个金针菇栽培菌株中获得13种不同的黄色单核体菌株和3种白色单核体菌株;

26. Selection of New Strains of Rhizopus from Protoplast Culture

利用原生质体培养技术选育根霉新菌株

27. STUDY ON MATING REACTION OF NEUTER STRAIN IN PLEUROTUS SAJOR-CAJU BY PROTOPLAST FUSION

利用原生质体融合研究凤尾菇中性菌株的交配反应

28. The formation of the polarity of Lilium davidii Duch pollen protoplast and the dynamics of actin cytoskeleton were observed by non-fixation, Alex-phalloidin probing and confocal laser scanning microscopy.

利用改进的Alexphalloidin活细胞染色方法和激光共聚焦显微镜技术,观察川百合(LiliumdavidiiDuch)花粉原生质体极性形成及萌发过程中微丝骨架的列阵变化。

29. Keywords protoplast;fusion;optimums condition;

原生质体;融合;最佳条件;

30. Keywords protoplast;fusion;thermotolerant;high-alcohol-producing yeast;

原生质体;融合;耐高温;高产酒精酵母;

31. protoplast metabolism factor

原生质体代谢因子

32. protoplast maintenance medium

原生质体保养培养基

33. protoplast conservation culture

原生质体保持培养

34. Protoplast regenerating rate

原生质体再生率

35. Advance in Protoplast Technique and Its Application in Vegetable Breeding

原生质体培养及其在蔬菜育种上的应用

36. The Progress of Protoplast Culture Research On Brassica Vegetable Crops

原生质体培养在芸薹属蔬菜作物中的研究进展

37. protoplast cultural condition

原生质体培养条件

38. Generation of fungal protoplast is an essential tool for genetic transformation system.

原生质体是研究和建立真菌遗传转化系统的重要工具。

39. Mutagenesis - selection of Saccharomyces cerevisiae which have the better ability of reducing diacetyl by protoplast mutagenization with UV.

原生质体紫外诱变筛选还原双乙酰能力强的啤酒酵母。

40. protoplast fusion technology

原生质体融合

41. Keywords protoplast fusion;strain breeding;PVA degradation;

原生质体融合;菌种选育;PVA降解;

42. Keywords protoplast fusion;wine;acid-degradation;

原生质体融合;葡萄酒;降酸;

43. Keywords protoplast fusion;fusant;S.cerevisiae;S.diastaticus;haploidy;

原生质体融合;融合子;酿酒酵母;糖化酵母;单倍体;

44. Keywords Protoplast fusion;Fusant AY068;High temperatural ethanol fermentation;

原生质体融合;融合株AY068;高温乙酵发酵;

45. Protoplast fusion technique and its application in feedstuff development

原生质体融合技术在饲料开发中的应用

46. The protoplast fusion technique originally appeared in 1970s has been greatly developed in recent years and shown out huge potential in microorganism breeding.

原生质体融合技术是20世纪70年代发展起来的一项新技术,在微生物育种方面显示出了巨大的潜力。

47. Construction of high palmitoleic acid-producing yeast by protoplast fusion

原生质体融合技术构建棕榈油酸高产酵母菌株

48. The Breeding of High Efficient Strain with the Technique of Protoplast Fusion to Degrade Polyvinyl-Alcohol

原生质体融合技术选育高效菌株降解聚乙烯醇

49. Construction of Novel Yeasts for Alcoholic Beverage By Protoplast Fusion

原生质体融合构建新型酿酒酵母

50. Constrution of Thermotolerant Alcohol Producing Yeast by Protoplast Fusion

原生质体融合构建耐高温酵母菌株

51. Protoplast Fusion to Construct Acid-Degradation Wine Yeast

原生质体融合构建葡萄酒降酸酵母的研究

52. Construction of Antagonistical, Insecticidal and Endophytic Multifunctional-engineering Strains by Protoplast Fusion

原生质体融合构建防病、杀虫和内生多功能工程菌

53. Construction of High-Efficiency Degradation Engineering Microorganism from Protoplast Fusion

原生质体融合构建高效降解工程菌的研究

54. Studies on Construction of Aroma-improving Yeast Stains for Cider by Protoplast Fusion Method

原生质体融合法构建增香型苹果酒酿造酵母的研究

55. Grape Wine Yeast by Protoplast Interfusion Techniques to Decrease the Acidity of Grape Wine

原生质体融合葡萄酒酵母用于葡萄酒降酸

56. protoplast isolation technique

原生质体隔离技术

57. Protoplast The protoplasm and plasma membrane of a cell after removal of the cell wall, where present. This can be achieved by physical means or by enzymic digestion.

原生质体：指去掉细胞壁以后的细胞原生质以及质膜。可用物理方法或酶消化得到。

58. Keywords Bifidobacterium;Saccharomyces cerevisiae;protoplast fusion;fusant;

双歧杆菌;酿酒酵母;原生质体融合;融合子;

59. There are usually horizontal rather than the slanting end walls of tracheids.Vessel elements have lignified secondary cell walls and at maturity lack a living protoplast.

另外，导管分子具有木质化的次生细胞壁结构，成熟期的细胞内已经不存在活的细胞质。

60. On the other hand, using the protoplast fusion technique, rhizobia strains of different category and species were fused to breed new genetic engineer strains possessing wide inoculation.

同时，利用原生质体融合技术进行根瘤菌的属间及种间融合，选育结瘤范围更广的遗传工程新材料。

61. Studies on fermentative characters of a brewer,syeast protoplast fusant GR8

啤酒酵母融合株GR8发酵特性的初步研究

62. The pilot fermentation test of brewer,s yeast protoplast fusant GR5

啤酒酿造酵母原生质体融合株GR5的中试

63. Keywords killer yeast;cider yeast;killer plasmid;protoplast fusion;

嗜杀酵母;苹果酒;嗜杀质粒;核融合缺陷;原生质体融合;

64. Keywords Killer yeast;Killer plasmid;UV. killing protoplast;Fusion;

嗜杀酵母;质粒;紫外线致死原生质体;融合;

65. So lower POX and SOD activity, lower contents of soluble protein and amino acid in Actinidia calluses were related to the diffculties in their protoplast culture.

因此较低水平的POX、SOD活性以及可溶性蛋白质和氨基酸含量是两种猕猴桃子叶愈伤组织原生质体分裂率低和玉米幼叶难以培养成功的影响因子。

66. Therefore adopted 3:1 as the best inoculation proportion of NU-1 and NU-2.The effects of different factors on protoplast formation of Bacillus thuringiensis strain NU-2 were studied .

因此采用3:1为NU-1、NU-2混合发酵的最佳配比。

67. Protoplast Culture of Horticultural Plants and Its Application: A Review

园艺植物原生质体培养及应用

68. It was favour for the improving of protoplast vigour to the medium with 2mg/L Gly, 100mg/L Glu, 300mg/L LH, 100mg/L yeast extraction and 500mg/L BSA.

在悬浮培养基中加入2mg/L的甘氨酸，100mg/L的谷氨酸、300mg/L的水解乳蛋白、100mg/L的酵母提取物、500mg/L的牛血清白蛋白有助于原生质体活力的提高。

69. An axial filament, with a similar structure to a bacterial flagellum, is spirally wound about the protoplast inside the cell wall.

在细胞壁内部，和细菌鞭毛有相同结构的轴向细丝包绕细胞质并使之螺旋化。

70. Saccharomyces cerevisiae TK15-94-33 (a lys" phe") and Saccharomyces kluyveri TK63-41-72 (a ade" leu") were screened as parent strains. Using protoplast fusion technique, a fusant TKF37 with high erogsterol content and biomass was obtained.

在选育麦角甾醇高产菌方面，以啤酒酵母TK15-94-33(a lys~- phe~-)和克鲁弗酵母TK63-41-72(a ade~- leu~-)作为亲株，运用原生质融合技术，得到一株麦角甾醇含量和生物量都较高的菌株TKF37。

71. Keywords Spinosads;Saccharopolyspora Spinosa;Breeding protoplast;medium;screen;

多杀菌素;刺糖多孢菌;育种;原生质体;培养基;筛选;

72. Optimization of the System of Protoplast Culture and Regeneration for Chinese Cabbage

大白菜原生质体培养再生体系的优化

73. The regeneration system of soybean is not perfect because the differentiation of callus tissue and the protoplast regeneration are difficult .

大豆因其愈伤组织难以分化、原生质体再生困难等因素,其再生体系一直不够完善。

74. The result showed that the protoplast of rhizopus and candida can merge each other.

实验发现根霉和酵母有融合的可能。

75. The method of protoplast fusion was proved a simple and efficient way to improving the product of itaconic acid.

实验结果表明,这种方法既简易又有效。

76. The characteristics of K+ uptake kinetics of four flue-cured tobacco genotypes were studied and their whole cell transmembrane inward K+ current of root cortex protoplast were recorded.

对4个烤烟基因型进行了根系钾吸收动力学特性研究,并对其根皮层细胞质膜内向跨膜钾电流进行了全细胞记录。

77. Keywords Trichoderma koningii;protoplast;mutagenesis;corn straw;alcohol;

康氏木霉;原生质体;诱变;玉米秸杆;酒精;

78. Application of He-Ne Laser in Heterogeneous Protoplast Fusion[J].

引用该论文 朱振华,胡欣荣,陈五岭,陈亮.

79. When the "+" and of "-" meeting of the mycelium, in the relative position can be put in Ascomycetes, then the relative distribution of the different mating type Ascomycetes the protoplast fusion.

当“+”系和“-”系菌丝相遇，在相对的位置可以产生原配子囊，而后相对的不同交配型的配子囊的原生质体融合。

80. Keywords Mikania micrantha;Roselle;protoplast fusion;cell growth;anthocyanin;

微甘菊;玫瑰茄;原生质体融合;细胞生长;花青素;