transfection
transfection
1. The adhesion rate was decreased by 11.34% at 3 h after transfection, and by 24.00% at 4 h after transfection, but the cloning efficiency did not change much.
3h及4h的细胞粘附率分别下降11.34%和24.00%,克隆形成率则没有明显变化。
2. The result shows that the expression of EGFR of BGC823 cells can be inhibited by transfection of CKLFSF8.The novel chemokine may has a bright prospect in the treatment of tumor.
CKLFSF8基因转染对人胃癌细胞株BGC823EGFR的表达有明显抑制作用,可望在肿瘤治疗中发挥重要作用。
3. The double FITC-UEA-I and Dil-ac-LDL fluorescence staining displayed differentiated EPCs. The expression of VEGF was found in the medium of EPCs transfected with pcDNA3hVEGF165.VEGF165 gene transfection could stimulate EPCs proliferation.
FITC-UEA-I和DiI-ac-LDL荧光双染证实分化的EPCs,脂质体介导pcDNA3-hVEGF165质粒转染组EPCs培养基上清中表达VEGF,hVEGF165基因转染促进EPCs增殖,注射转基因EPCs的大鼠皮下局部血管增加。
4. Transient transfection shows that GCRG213 is located in cytoplasm and nuclei of COS-7 cells.
GCRG213蛋白在胞核和胞质中均有表达。
5. The proliferation capability K562eGFP-bcr cells was inhibited at 48 hrs after transfection and decreased significantly 72 hrs later.
K562eGFP·bcr细胞在体外培养48小时后增殖开始下降，72小时后细胞增殖下降较明显。
6. Transfection of LRP AsODN into A2780/DDP cells down-regulated the LRP expression and reversed the resistance phenotype to cisplatin.
LRP AsODN能明显降低A2780/DDP细胞中LRP表达水平,逆转其顺铂耐药表型。
7. The influence of NT-3 transfection in vitro on the growth of mice cochlear spiral ganglion cells[J].Chin J Neurosurg Dis Res,2004,3(3):249-251.
NT-3基因转染对小鼠耳蜗螺旋神经节细胞生长的影响[J].中华神经外科疾病研究杂志,2004,3(3):249-251.
8. After transfection, the expression of CD151 of p-AAV-CD151 in transfected group significantly increased compared with non-transfected group and p-AAV-GFP transfected group(P<0.05).
p-AAV-CD151组CD151表达明显增加,与正常对照组、p-AAV-GFP组比较表达差异有统计学意义(均P<0.05)。
9. After transfection with pcDNA3-p21, the proliferation of cultured BGC was significantly inhibited and cells in G1 phase were resisted.
p21导入胃癌BGC细胞后,肿瘤细胞增值能力明显受到抑制,并出现细胞周期G1期阻滞。
10. By transfection, CHV1-EP721 was successfully introduced into virus-free fungal host strains EP721(-), EP155 and Euro7(-).
p721的体外转录产物成功转染无病毒的板栗疫病菌野生菌株EP721(-)、EP155和Euro7(-)。
11. RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression.
RT-PCR结果显示:E1A基因在Hela细胞中能稳定表达,E1A基因的转染明显降低了Her-2基因在Hela细胞中的表达水平;
12. RT-PCR demonstrated that E1A gene had a stable transfection in Hela cells and obviously down-regulated HER-2/neu expression.
RT-PCR结果显示:E1A基因在宫颈癌细胞中能稳定表达并且显著降低HER-2/neu在宫颈癌细胞中的表达水平。
13. Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression.
Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明，外源的Stat3蛋白具有DNA结合活性，并可增强报告基因的表达。
14. The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene.
Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
15. TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer.
TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
16. TUNEL staining showed typical apoptosis characteristics of E10 cells after transfection.
TUNEL染色显示，E10细胞出现典型的凋亡特征。
17. ULBP3 gene transfection make Saos-2 cell line more sensitive to human NK killing.
ULBP3基因转染能提高人外周血NK细胞对人成骨肉瘤Saos-2细胞的杀伤活性。
18. Western blot showed that protein lev els of Ang1and VEGF 165 were in-creased 3.53and 11.53fold respectively 24h after transfection as com-pared to control.
VEGF165和Ang1蛋白表达分别为对照组的11.65倍和3.53倍。
19. In both transfection and infection models, irus particles were released in a cyclical manner, with bursts of irus produced eery 10-14 days.
上述两种转染和感染模型中，发现病毒颗粒呈周期性复制，一般10-14天增加一倍。
20. To examine the function of STAT-JH1, we performed transfection study in COS-1 cells.Immunoblotting of cell lysate showed that the product of STAT-JH1 could undergo auto-tyrosine-phosphorylation.
为进一步了解他们的蛋白质特性，分别将五种基因转染到COS-1细胞，由西方墨点法分析，STAT-JH1融合蛋白能够自行进行酪胺酸磷酸化作用；
21. The recent progress of usign magnetic manoparticles in gene transfection is introduced. Present difficulties and future prospect are also included.
介绍了磁性纳米颗粒介导基因转染的最新研究进展,面临的主要问题以及将来的发展方向。
22. The transfection efficiency of rAAV1/2-lacZ vector gene from umbilical mesenchymal stem cells cultured in vitro is satisfactory and becomes higher as MOI increased.
体外培养的脐血间质干细胞能高效转染重组腺相关病毒1/2载体-lacZ报告基因,在一定感染复数范围内,细胞转染率随着感染复数的增加而升高。
23. The gene transfection experiment in vitro showed that the pDNA-chitosan nanoparticles can transfect BHK celles , and the gene can express in these celles.
体外基因转染实验表明壳聚糖作为一种新型的非病毒基因递送载体能够高效传递DNA进入BHK-21细胞，基因能够在该细胞中高效表达；
24. Transfect PK-15 cells with SK-PCV2-1(DB) in vitro. After four passages,the infectivity of the cloned chimeric PCV2-1 genome was confirmed by RT-PCR and IFA.
体外转染PK-15细胞,盲传4代后,用RT-PCR和间接免疫荧光方法检测,结果表明本试验构建的嵌合病毒PCV2-1克隆具有感染性。
25. As an important technique in biochemistry and molecular biology, transfection is meaningful for its potential applications in gene therapy.
作为现代生物化学和分子生物学中的一种主要技术手段，转染对于基因治疗有重要的意义。
26. CONCLUSION: Transfection of Kv1.5 AsOND made the function of Kv in HASMCs decreased. Kv1.5 may play a critical role in the regulation of Kv activity.
使细胞膜电位(Em)趋向于去极化方向。 结论:转染Kv1.5 AsOND可导致HASMCs Kv功能降低,Kv1.5基因亚型在调节Kv活性中可能起重要作用。
27. Immunohistochemical staining showed that after mouse NIH3T3 cell transfection for 4 days positive expressive products appeared in cytoplasm, and control group showed negative result.
免疫组织化学染色显示:小鼠NIH3T3细胞转染4d后胞浆内可观察到阳性表达产物,对照组呈阴性结果。
28. The study of gene transfection into rabbit's tongue muscle in vivo and optimization.
分子外科缝线术的建立及其优化方案的研究。
29. Using methylation specific PCR (MSP) to detect the CpG island methylation changes of PRA & PRB promoters in the leukemia cell lines before and after 5-Aza CdR treatments and the MG88 transfection.
利用MSP检测白血病细胞株在5-Aza CdR处理前后、MG88的转染前后PRA、PRB启动子CpG岛甲基化状态。
30. The expression of RTN in HCC were observed by immunohistochemical staining, eukaryotic transfection tumorigenicity.
利用免疫组化、真核转染、体内外抑瘤试验等方法。
31. Bacteria can be transformed with DNA extracted from a bacterial irus rather than from another bacterium, a process known as transfection.
原核生物中，偶尔也用到 transfection 这个词，当细菌的转化过程中吸收的外源DNA是病毒DNA时就应该称此过程为转染。
32. Rearrangements and mutations involving the RET(Rearranged During Transfection) proto-oncogene have been implicated closely in the papillary thyroid carcinoma(PTC).
原癌基因重排及突变与甲状腺乳头状癌(PTC)密切相关,它在调节细胞增殖、迁移、分化过程中具有重要意义。
33. Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs.
反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
34. At the same time, viral vectors are recombined and antiangiogenesis is adopted, which makes transfection into gliomas more efficient and selective.
同时对病毒载体进行重组,对肿瘤血管生成的抑制等实现选择性地、高效地对肿瘤组织的转染。
35. The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry.
同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
36. Meantime, the qualitative observation demonstrate that the proliferation of the smooth muscle cells with the eNOS gene transfection was inhibited through the NO.
同时，实验通过定性观察验证了转染eNOS基因可以通过产生NO，抑制血管平滑肌细胞的增殖。
37. Polymeric carrier containing folic acid is expected to be able to identify tumor surface receptors and transfect cells by receptor-mediated endocytosis.
含有叶酸的聚合载体能够识别肿瘤表面受体，并且能够通过受体介导的(细胞)内摄作用转染细胞。
38. Mesenchymal stem cell has the potential for application because of it s characteristics of isolated and amplificated easily、gene transfection、haematopoiesis and immunoregulation.He...
因间质干细胞具有易分离扩增、基因转染、支持体外造血、体内造血重建以及免疫调节作用等特点,在临床上有很大的潜在应用价值。
39. With FuGENE6 transfection reagent, AIRE gene was stably expressed in HeLa cells that would be helpful for further study on AIRE gene.
在 Fu GENE6转染试剂的介导下 ,AIRE基因在 He La细胞内获得稳定表达
40. In the optimum condition: FuGENE 6:DNA=6:1, transfection of rat MSC withGFP displayed a stable and long-term GFP expression in vitro, and untransfectedMSC gradually died in G418 selecting culture.
在FuGENE6:DNA二6:1的优化条件下,MSC能在体外长期稳定的表达绿色荧光蛋白,未转染上的细胞在G418的筛选下逐渐死亡。
41. In transfection group of N2a cell line, CDK5 expression increased presented by deep coloration of SMI31 antibody and weak coloration of SMI32 antibody, implying hyper-phosphorylation of neurofilaments.
在N2a细胞株转染组中,细胞周期蛋白依赖性蛋白激酶-5表达增加,并使抗体SMI31显色增强,SMI32显色减弱,提示神经细丝被过度磷酸化。
42. The transfection and expression efficiency of RV was above 30% in SK-N-SH cells determined by observing EGFP expression.
在SK-N-SH细胞EGFP的荧光表达显示RV的转移并且表达的效率达到30%以上。
43. MSCs were transfected with enhanced green fluorescent protein (EGFP) gene under the mediation of LipofectAMINE, and G418 resistance was adopted for screen, so as to observe the transfection efficiency.
在脂质体介导下,将增强的绿色荧光蛋白基因导入骨髓间充质干细胞,然后通过G418抗性筛选,观察转染效率。
44. Transfection with full-length VP15 resulted in GFP fluorescence being distributed exclusively in the nucleus.
在转染全长VP15基因的细胞里，荧光信号只分布在细胞核内。
45. The aim of gene therapy is to transfect DNA into target cells, in which ultilizing antisense oligonucleotides is a main method.
基因治疗是将DNA转染进入目的细胞,修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。
46. Gene transfection with IkB can inhibit the local and total inflammation effectively.
基因转染可以有效抑制鼠肝和系统的炎症过激反应。
47. Gene transfection is a technology that heterologous genes transfect eukaryotic cells, this article will summarize the significance, classification, methods and application of transfection.
基因转染技术是将外源基因转染真核细胞的一种技术,本文将对外源基因转染真核细胞的目的意义、转染技术分类、方法及其应用等做一综述。
48. Gene transfection represents a important technology in the molecular biology and for the gene therapy.
基因转染是分子生物学领域中一项重要技术,也是实现基因治疗的前提条件。
49. Gene recombination and gene transfection are the new progresses in molecular biology,which provides theory and technology basis for further study of tissue engineering.
基因重组、基因转染是分子生物学研究的新进展,为组织工程的深入发展提供理论和技术基础。
50. Transcription level of HPV E6 gene decreased significantly, the amount of P53 protein increased significantly and the apoptosis rate of Ca Ski cells increased significantly after hIL-24 transfection.
处理后Ca Ski细胞中HPV E6癌基因的mRNA水平下降,抑癌蛋白P53水平增加,细胞凋亡率升高.
51. Exogenous hTERT gene transfection could promote the proliferative capacity of hEF.
外源性hTERT基因转染可使hEF增殖能力增强。
52. Nanoparticles and DNA conjugation have been used widely in drug delivery, gene therapy and gene transfection.
奈米颗粒与DNA的结合有广泛的用途，如药物投递、基因治疗、基因转染等。
53. The study observed the transfection efficiency of recombinant adeno-associated virus with enhanced green fluorescent protein transduction to rat bone marrow mesenchymal stem cells in vitro.
如果进一步在基因水平上分析转染后的骨髓间充质干细胞有哪些生物学特性，如外源基因的表达、细胞凋亡的变化等，实验结果会更加完善和系统。
54. Keywords ONDs;transfection;cationic liposome;
寡核苷酸;转染;阳离子脂质体;
55. This study mainly deals with cell transfection and cytotoxicity for PEI(10kD)-PBLG, a novel cationic copolymer, to observe its potential as a gene carrier.
对新型阳离子聚合物PEI(10kD)-PBLG进行研究,重点考察其基因转染效率与细胞毒性,探讨其作为基因载体的可能性。
56. Transgenic chicken could be obtained by transfection the PGCs in vivo or in vitro and screening the offsprings.
对鸡PGCs进行体外或者体内转染外源基因操作，再通过后代的筛选，就能获得转基因的后代。
57. It is a hot point for gene therapy to find a safe and high-efficient gene transfection too1.
寻找高效、安全的基因转染方法一直是基因治疗的热点问题。
58. These clones were used to transfect fetal donkey dermal (FDD) cells and donkey differentiated monocyte-macrophages (DL), and their infectious characteristics were monitored by RT-PCR and reverse transcriptase activity assay.
将所改造的突变克隆转染驴胎皮肤细胞(FDD)以及驴单核巨噬细胞(DL),并用逆转录酶活性检测和 RT PCR方法验证其感染性。
59. In addition, the interaction between matrix m etalloproteinases (MMPs) and ET-1 was investigated by cel l transfection with anti-ET-1 converting enzyme (anti-ECE) mRNA expression plasmid followed by gelatin zymography analysis.
将细胞转染反义内皮素转换酶(anti-ECE)mRNA表达载体并运用酶谱分析法,研究ET-1与基质金属蛋白酶(MMPs)的相互作用。
60. The results of testing by Western blotting showed an expression fragment in supernatants of pSecTag2 ES transfected COS 7 cells at 48 and 72 hours of transfection.
将该 ES c DNA重组到真核表达载体 p Sec Tag2 / Hygro B上 ,转染 COS- 7细胞后 ,用Western blotting法检测。
61. The recovered bands of PrP, GFP and pCDNA3.1(-) were ligated with T4 ligase to produce a fusion expression plasmid, pCDNA3.1(-)-mPG, which was then used to transfect the Neuro-2a cell under the selection pressure of G418 antibiotics.
将这三个片段共连接,从而获得融合mPrP/GFP基因的真核表达质粒pCDNA3.1(-)-mPG。 由于在PrP C-端融合了绿色荧光蛋白(GFP),使该系统具备了早期筛选的标签。
62. The inhibitory effect of gene transfection on solid tumor was observed in mice.
小鼠实体瘤块模型研究基因转染抑制肝癌生长作用。
63. Keywords DsRNA;Interferon;Transfection;Apoptosis;
干扰素;转染;凋亡;
64. The positive clones were screened by G418.The proliferation,migration,and invasion of MDA-MB-231 cells before and after transfection were assessed by CFSE-flow cytometry,monolayer wound healing assay,and soft agar colony formation test.
应用CFSE-流式细胞仪检测,单层伤口愈合实验,软琼脂克隆形成实验等方法观察了MDA-MB-231细胞转染前后,细胞体外增殖及侵袭能力等指标的变化。
65. RT-PCR,CFSE-flow cytometry and monostratal wound healing were used to determine the expression levels of PI4K and AKT,cell proliferation and motility of the SKOV-3 cells,respectively,before and after the transfection.
应用RT-PCR、cfse-流式细胞测定和单层伤口愈合实验等方法观察转染前后SKOV-3细胞PI4K和AKTmRNA表达水平,细胞增殖及其运动能力变化。
66. Overexpression of wild-type Ras enhanced CpG ODN-induced ERK, JNK and NF-xJB reporter gene activation, On the contrary, RasN17 transfection inhibited CpG ODN-induced ERK1/2 and JNK1/2 phosphorylation and NF-KB reporter gene activation.
我们用免疫沉淀实验发现CpG ODN还能诱导Ras与TLRg的结合。 同样条件下non一CpG既不能活化Ras，也不能诱导Ras/T LRg复合物的形成，提示Ras活化以及Ras与TLRg的结合具有CpG基序特异性。
67. In the present study, the effect of DOC - 1R expression on DNA replication and centrosome duplication was investigated using plas-mid DNA transfection and dual immunofluorescent microscopy.
我们结合转染技术及间接免疫荧光技术，观察细胞内DOC-1R转染基因表达对于DNA复制以及中心体复制的影响。
68. The effect of 12C6+ beam irradiation on AdCMV-GFP(a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated.
探讨12C6+离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒(AdCMV-GFP)转染小鼠黑色素瘤细胞(B16细胞系)的影响。
69. FnCBD_(64) transfection efficiently promote adhesion of EPCs on decellularized xenograft aortic valves. Still, much work is needed in the future study of TEHV.
携带信号肽序列的Ad. FnCBD_(64)转染EPCs可有效提高细胞的粘附力,有助于TEHV的构建,但目前方法构建的TEHV还不能耐受大流量流体的冲刷,未来TEHV的研究还有很多问题需要解决。
70. Abstract： Objective： The aim of this study was to investigate vitro/vivo killing effect of suicide gene CD on the human gastric cancer cell line 7901 by gene transfection method.
摘 要： 目的：通过转基因方法观察自杀基因CD对人胃癌细胞株7901的体内外杀伤作用，并观察组织病理变化。
71. DNA condensation is a common physiological phenomenon in nature and also a key step in non-viral vector-mediated gene transfection.
摘要DNA缩合不仅是自然界常见的生理现象，也是非病毒载体介导基因转染的关键步骤。
72. Gene transfection is the key technique of gene therapy.
摘要基因治疗的关键技术是基因转移。
73. This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system.
摘要本研究旨在提高基因转染人红白血病细胞株K562的转染效率，同时观察绿色荧光蛋白(GFP)作为报告系统用于转染条件优化的可行性。
74. This study was aimed to investigate the transfection efficiency of adenoviral vector AD5/F35 to hematopoietic malignant cells lines of various origins and AD5/F35 cytotoxicity.
摘要本研究比较AD5/F35腺病毒载体对不同来源的血液系统恶性细胞系感染效率的差异和细胞毒性反应。
75. Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
76. Adenovirus as gene transfer vector is characterized by high transfection efficiency, high titer, good stability in target cells and low pathogenicity, and has been widely used in gene therapy.
摘要腺病毒载体是一种非常有效的转基因载体，具有高效率、低致病性、高滴度及不整合入宿主细胞染色体等优点，广泛应用于基因治疗。
77. Abstract： One of the most important challenges of tumor gene therapy is to develop safe and effective gene transfection Carriers.
摘要： 肿瘤基因治疗的最大挑战之一是研制安全有效的基因转染载体。
78. The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection.
收集转染前、转染后24 h和48 h的毛滴虫细胞,用半定量反转录-PCR(RT-PCR)检测Prx和TrxR的mRNA水平;
79. Larger (200-250 nm) and smaller (50 nm) nanoparticles were compared to evaluate the effects of size on transfection efficiency as well as any associated intraarticular reaction.
文中比较了大（200-250nm）、小(50 nm)颗粒的结果，希望以此了解尺寸效应对转染率及其它相关的关节内反应的影响。
80. Methods: Eukaryotic expression vector plasmid containing AIRE cDNA was transfected into HeLa cells with FuGENE6 Transfection Reagent.
方法 :用 Fu GENE6转染试剂将含 AIRE c DNA的真核表达载体转染 He La细胞 ,经 G41 8筛选阳性克隆。
